【之哺乳类细胞的信号转导通道与非定标性突变形成的相关性】.pdf
哺乳类细胞的信号转导通道与 非定标性突变形成的相关性 浙江医科大学病理生理学 95级硕士生:戴群 导师:余应年陈星若 摘要 细胞受外源性DNA损伤因子作用而导致的遗传不稳现象,表现为迟发的 点突变,微卫星序列不稳,染色体重排等,是生物进化、遗传病发生及肿瘤形 成的起点,亦为基础理论研究的热点。非定标突变是相对定标突变而言的,表现为迟发和延续的在DNA直接受外源损伤因子功击部位之外的基因突变.本实验室曾以穿梭质粒为探针巧妙地诱导了DNA烷化剂一甲基硝基亚硝 胍(MNNG)作用于猴肾Vero细胞引起的非定标性突变。
TheParticipation of Cell SignallingPathway in Nontargeted Mutagenesisin MammalianCells postgraduate for M.A.in pathophysiology:Dai Qun Supervisors:YuYingnian,ChenXingruo ZhejiangMedicalUniversity ABSTRACT 1.Background Genetic instability,appeared asdelayed onset of genepoint mutation,microsatellite
procedure of experiment with addition of PKAinhibitor PKIwas similar tothatof tyrosineinhibitorexceptthatVerocells were treatedwithcellpermeatants after prior treatmentwithMNNG or DMSO.IV.Results In the tyrosine kinase inhibition experiment,the mutation frequency of group M,D,MG,DG were 14,3.