【硝苯□啶的异丙肾上腺素对平滑肌细胞连接通讯的影响】.pdf

【硝苯□啶的异丙肾上腺素对平滑肌细胞连接通讯的影响】.pdf

样硬化时平滑肌细胞生长调控机制及其防治提供理论依据.本文采用贴块法分离新生大鼠主动脉的平滑肌细胞,是在以往本 实验室用同样方法培养的平滑肌细胞经电镜下证实有丰富的微丝束、免疫组化提示为肌动蛋白的基础上进行的,根据光镜下细胞外形呈极 形、生长方式皇“藓谷”样,从而确定为平滑肌细胞。本研究使用5 -16代的平滑肌细胞。当平滑肌细胞生长汇合成单层时,分别加入硝 苯吡啶、异丙肾上腺素、佛波酯等药物.通过荧光黄划痕示踪法了解硝苯吡啶、异丙肾上腺素、佛波酯等 药物对平滑肌细胞连接通讯功能的影响。由于荧光黄(Lucifer Ye11ow)CH分子量为457.TheEffectsofNifedipineandIsoproterenolOn The SmoothMuscleCellssJunctional Communication Department of Pathology(Cardiovascular Disease) Zhejiang Medical University PostGraduate94Xue-songBai Tutor Professor Ding-anLou Abstract 1tis intensivelyadmiredthatGapJunctionbetweentwo contacted cells is the saortausingpiecesattachedtreatmentandidentifiedby cellmorphology,growthpattern throughelectron microscope andimmunhistochemistry.Themethodcalled scrapeloadingdye-transferwasused in our experiment to test the junctionalcommunication.As akind of dye,Lucifer YellowCH(Mw457.