【水稻第3号染色体物理图谱构建】.pdf
目录 致谢 中英文摘要 综述 1 前言-9 1 真核基因组DNA的分析和克隆 9-30 1用于构建真核基因组DNA文库的载休 1基因组分析常用技术 1 模式生物的基因组结构分析-45 1大杆菌 1酵母 1拟南芥菜 1线虫 1 人基因组研究计划 45-50 1 水稻基因组研究计划 50-55 实验 第一部分BAC库的指纹图谱制作和contig构建 实验方法与步骤.
Abstract The strategyofconstructionof thechromosome3physicalmapof rice(Oryza sativa)based on anchor scts by fingerprinting techniques is described.BAC contigs in the physical map covers 77 of the rice chromosome3.BAC contigs arepositioned along the chromosoime 3by screeningBAC librarywith66anch
ctal,1994),STS markers were produced by PCR based on the publication(T.lnoue elal,1994)BAC librarywere grided on 9 Ilybond Nmembranes(5x5x96) using BIOMEC 2000,then screened bymarkers,onthewhole233BACcloneswerefound,noBACclonewas foudformarkers.The average number of4.