【大麦黄花叶病毒单克隆抗体的制备及McAb-ELISA检测BaYMV方法的建立】.pdf
目录 摘要.ABSTRACT 前言 材料与方法 一、BaYMV单克隆抗体的制备及性质鉴定(一)小鼠免疫 1、病毒来源及分离纯化 2、小鼠免疫(二)细胞融合及杂交瘤的筛选 1、骨髓瘤细胞系和饲养细胞 2、培养液及选择培养液 3、细胞融合 瘤 4、杂交的筛选 5、杂交瘤的克隆化(三)单克隆抗体及多克隆抗体的制备 1、小鼠腹水的制备
综述 单克隆抗体在植物病毒研究中的作用 致谢 ii
ABSTRACT The BALB/C mouse sepleencytes immunized with Barley Yellow Masaic Virus(BaYMV)and mouse myeloma(NSl) were fused by PEG The hybridomas were selected by indirect-ELISA and limiting dilution methods.Four kinds of stablehybridoma cell lines which can secreting monoclonal antibodies(McAb) were