【应用花药培养产生抗除草剂转基因水稻纯化合体】.pdf
PRODUCTIONOFHOMOZYGOUS HERBICIDE-RESISTANTTRANSGENICRICE VIAANTHERCULTURE ADissertation Presented to the Faculty of the Graduate School of Zhejiang Agricultural University inPartialFulfillmentoftheRequirementsforthe DegreeofMasterofScience by PingLuo June1997
MS培养基上分化成苗。不同氮气压力条件下,分化率有所变化,1300 psi压力条件下,分化率最低,可能压力大,对悬浮细胞的损伤较大。花 粉管通道法转化水稻采用两种质粒浓度(0,1pg/10ul),并以TE缓冲液 结实率很低,最低为9 ,最高为38。结实率与导人时间有关,一 般导人时间晚,结实率高。可能导人时间早会影响水稻的正常受精。
Inheritanceofbarin theDHpopulationswasinvestigated.In most of the DH populations the segregation pattern of bar expressionfit a1:1 ratio,as expected by a single dominantgenesegregatinginaMendelianfashion.However,irregularinheritanceoftransgenewasfoundin threeDHpopulations.