[联免疫吸附测定法检测乙型肝炎表面抗原的研究]

【联免疫吸附测定法检测乙型肝炎表面抗原的研究】.pdf

酶联免疫吸附测定法险测乙型肝类 表面抗原的研究,工.酶结合物的制备和质量鉴定 Ⅱ.ABSTRACT The peroxidase conjugates Composed of horse-radish peroxidase(HRP) and anti-HBs were prepared by using benzoquinonemethodandmodifiedperindatemethadres-pectively.The coupling efficiency of inodified pericdate conju-gation wos the best,7o of the added HRP wos Coupled with two-step metho材料和方法 辣根过氧化物酶一抗-HBs结合物的制备 辣根过氧化物酶(HorseradishPerxd:sHRD),洒蕊 BpehrineYMa比kheimGmbH一级产品,强疾际点减纯 体(Rz)A403Km/A280Km=3,按邓联香妆本洲时 酶活性为3584U/m,符合标记用酶的要求.戊二醛(Giutara1dehyde)E.MevcK出品,经测经A35nm/A280nm≤4, 过碘酸(SDd.periDdate,NaID)AR,上试剂二 厂出品,批号780302.翻氢化纳(Sod.borDhydride,NaBH4)类国A1dvicn化学 公同云品。