【日本血吸虫肾病的实验】.pdf
日本血吸虫肾病动物实验的研究(摘要)浙江医科大学传染病研究生 周维立 导师 王季午 马亦林 特异性抗在感染宿主肾腔中的定位及其在洗脱物中的存在是证实 血吸虫免疫复合物肾病的可靠依据。既往,国内外学者均用免疫荧光标 记技术检测肾胜切片中的抗、抗体和补体.本文首次应用深根过氧化物酶标记抗体间接染色法对感染2000 条尾购,50天剖杀的家兔肾脏切片中特异性抗作定位检测,同时用 琼脂双扩散法、间接血凝试验和酶联免疫吸附试验测定肾脏匀浆洗脱物 中的血吸虫抗院和抗译。
EXPERIMENTALSTUDYOFIMMUNECOMPLEX NEPHROPATHYOFRABBITSINFECTEDWITH S.JAPONICUM(Abstract) Postgraduat or Zhejiang Medical University:ZhouWei-li周继立)Instructors:WangJi-vu(王季午)MaYi-lin(马亦林)The presence oftheschistosomalantigeninkidney eluateanditslocalizationinthekidneysofinfected rabbitsaretheconfir
useofthepooledantiserumobtainedfromimmunized rabbits,it was formed that its specificity and de- siredtiterwerenotsatisfactory.Thus,theserum ofpatients with acute schistosomiasis in 1:20 dilu- vhilethehorseradishperoxidase(HRP)taggedgoat antihuman IgG diluted in 1:16 knom as enzyme-conjugate wasuseda