【绞股蓝提取液对人直肠腺癌细胞系Hce8693作用的实验研究】.pdf
绞股蓝提取液对人直肠腺癌细 胞系(Hce8693)作用的实验研究(摘要)近年来,许多研究表明,绞股蓝有明显的抗肿瘤作用,对培养 的肝癌胃癌、宫颈癌、肺癌等癌细胞均有较好的抑制作用,癌细 胞的增殖抑制卒为20一80 ,动物实验和临床试用于多种肿瘤 亦有较好的治疗效果。但至今为止尚未见到有关绞股蓝对大肠癌细 胞的作用及绞股蓝抗肿瘤作用的机制方面的实验报道 本实验以人直肠腺癌细胞系(Hce8693)和人胚肺二倍体成纤 维细胞材(4BS)为材料,进行绞股蓝提取液抗肿瘤作用的体外研 究,目的即在于观察绞股蓝提取液对人直肠腺癌细胞和正常成纤维 细胞的学响。
Research on effects of extract of Gynostemmau pentaphyllum on.human rectal adenocarcinoma cell line(Hce 8693)(abstract) In recendyoars,it has been showed that Gymostemma.pentaphyllum hasanantineoplastic effect。It can inhibit effeetively the growthiof cancor cells in culture of hepatic carcinema,ga
method respectively.The result wasexpressed in Cpm/ugprotein.Secondly,tho cells growm on the cover slips wore exposed to the extract with tho same concentrations and times as above,then fixed,stained with H.BThe cell morphologic changeswereobservedundermicroscopeand the cleavage indexes(CI)in 48h