【建立简捷塑料条ELISA方法诊断吸虫病及其应用的研究】.pdf
题目:建立简捷塑料条ELISA方法诊断 血吸虫病及其应用的研究 Developmentandapplication offastplasticstrip ELISA(PS-ELISA and FPS-ELISA)forSerodiagnosisof Schistosomiasis.
Abstract of the most sensitive,specific serodiagnosis tests and is broadlyappliedon Schistosomiasis.Owingtothe complicated procedures and higher cost,the application of ELISA in endemic field is limited.This research,based on the basic principle of ELISA,substitutes PVC plastic strip for microtitre
agher enzyme conjugated concentration isable to helpto educe the incubation time,so as to saving the total time fthe detecting.The substrate(oPD) incubation time is also studied,and the result showes that the sufficient conversionneeds atleast 30minat 37C.