【人胎盘抗凝蛋白变体的表达纯化及活性研究】杨翔.pdf
学 论文答辩日期:2070年5月15日 华春 指导教师:(签字)
ABSTRACT An AnnexinV-Hirudin fusion protein was contructed.The Gly-Pro-Arg-Pro was add to theN-terminus ofAnnexinVsoas toincrease thefibrin specificity,at thesame time the C-terminus17amino acid of Hirudinwas add to theC-terminus of AnnexinV.
Solution I:50mM Glucose,25mMTris-Cl,10mM EDTA(pH8) SolutionI1:0NNaOH(用前由1ON的NaOH稀释),1 SDS SolutionIll:3M Potassium acetate,5MGlacialaceticacid 制备感受态细胞所用试剂:Buffer T1:10mM,50mM MnCl.,10mMNaAc(pH 5) BufferT2:75mMCaCl,100mMMncl,10mMNaAc(PH5) SDS-PAGE所用试剂:A.