【活性氧对神经细胞的损伤及Ebselen的抑制】易永.pdf

ROS Damage Neuronal Cells And TheProtectionOfEbselen Ph.D.Candidate:Yi Yong Supervisor:Prof.Xu Huibi AssociateProf.YangXiangliang ADissertation Submitted to theDegree Evaluation Committee of Huazhong University of Science and Technology for theDegreeofPhilosophy in Biomedical Engineering December,

而与胞内钙库释放无关。此外,过氧亚硝基阴离子对[Ca2]的影响在一定程度上可被DTT(1mmol/L)抑制,而TTX(1μmol/L)无明显作用,说明它激活L-型钙离子通道是非电压依赖性的,可能与其氧化修饰L-型钙离子通道蛋白有关.5.Ebselen有效拮抗过氧亚硝基阴离子及超氧阴离子自由基以上各种神经毒性.关键词:谷氨酸兴奋性毒性过氧亚硝基阴离子超氧阴离于白由基胞内游离钙离子浓度(Ca1)Ebselen谷氨酸与其受体结合力电于自旋共振捕获和标记技术 Fura-2显微荧光测量技术

radicals arerelated to the damageof Na,K-ATPase.Theeffectofperoxynitriteon[Ca]:insingleMN9Dcellis studiedbyFura-2 microfluorometric techniquesecondsafterperoxynitrite application,[Ca2+];begins torise and then decreasesrapidlyback to thebasal level afterperoxynitriteremoval.