【红豆杉细胞悬浮培养生产紫杉醇优化研究】罗杰.pdf
Dissertation Submitted to theDegree Evaluation Committee of HuazhongUniversityof Science andTechnologyfor the DoctorDegree ofPhilosophy in Biomedical Engineering Optimizationof CultureStrategyandProcess ControlforPaclitaxelProductioninCell Suspension Cultures of Taxus Chinensis Ph.D.
华中科技大学博士学位论文 合成及释放的影响。红豆杉细胞悬浮培养过程中培养第12天添加2mg1硫酸铺 铵能获得最大紫杉醇产量8mg1,为对照组的4倍。同时添加2mg1硫酸铺铵、5 油酸(v/v)及20g1蔗糖时紫杉醇产量可达25mg1.5)研究了脱落酸对红豆杉细胞悬浮培养过程中细胞生长及紫杉醇合成的影 响。红豆杉细胞悬浮培养过程中培养第12天添加5mg1脱落酸能获得最大紫杉 醇产量11mg1,为对照组的5倍。同时添加5mg1脱落酸及20mg1茉莉酸甲 酯时紫杉醇产量可达18mgr.行了优化。
华中科技大学博士学位论文 phasefor14-daysfollowedbychangingtheDOlevelto20-40 duringthepaclitaxel production phasefor another 10-days.An optimum paclitaxel level of 7mg 1′ was obtainedby thedissolvedoxygencontrolledprocessbinationofnutrientsfeeding and dissolved oxygen tension control gavemaximum paclitaxel