[硒诱导结肠癌SW480细胞凋亡的信号转导机制研究]王海涛

【硒诱导结肠癌SW480细胞凋亡的信号转导机制研究】王海涛.pdfDissertation Submitted to AcademicDegreeEvaluation Committeeof HuazhongUniversityofScienceandTechnology For theDegree of Doctor of Philosophy in Biomedical Engineering Study on theMechanismsof Signal Transduction in Apoptosis of Human Colonic Carcinoma SW480 Cells Induced by NaSeO Ph.D.华中科技大学博士学位论文 强度下降并呈浓度和时间依赖性。钙通道抑制剂CoCl、胞内钙离子整合剂BAPTA 显著的抑制NaSeO诱导的膜电位的下降,同时可提高细胞存活率并降低NaSeO 细胞毒性。以上结果说明Ca在NaSeO诱导的细胞凋亡中起到信号转导作用.应用DCF-DA染色细胞的方法研究NaSeO,对胞内活性氧的影响,在激 光共聚焦显微镜下观察到NaSeO作用细胞2小时胞内ROS含量增加,随培养时 间的延长ROS含量将减少,作用6小时时恢复到基值水平。NaSeO诱导胞内ROS 含量增加呈浓度依赖性。华中科技大学博士学位论文 Effect of Na,SeO, on [Ca2], of SW480 cells wasstudied by observing scanningmicroscope.Theresults showed that[Ca2],was rapidly increased after cells were treated by NaSeO,(10-100 μmol/L) for 5 seconds.The fluo-3 fluorescent intensitywas3-foldof thatofrestingstate after100umol/LNaSeO
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